New PDF release: Advances in Forensic Haemogenetics: 14th Congress of the

By Prof. Dr. Christian Rittner, Dr. rer. nat. Peter M. Schneider (auth.), Prof. Dr. Christian Rittner, Dr. rer. nat. Peter M. Schneider (eds.)

ISBN-10: 3540551948

ISBN-13: 9783540551942

ISBN-10: 3642773249

ISBN-13: 9783642773242

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Additional resources for Advances in Forensic Haemogenetics: 14th Congress of the International Society for Forensic Haemogenetics (Internationale Gesellschaft for forensische Hämogenetik e.V.), Mainz, September 18–21, 1991

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The second standard finally contained 13 definable alleles (Fig. 3) but sometimes only 12 could be differentiated (Fig. 2). Figure 3 impressively demonstrates the problems with this type of electrophoresis. It can be seen that the separate DNA fragments in Iane A and B cannot be clearly assigned to the corresponding bands of the allelic ladder, although they are components of this ladder. PA gels cannot yet be produced with a unified quality so that the results are noi reproducible. It is necessary to obtain an even higher quality of PAG electrophoresis and resolution of fragments in this system from which a larger number of alleles can be assumed.

1991a). We have used the method to obtain 16 new STR loci. Each was identified in  clones. Our success at amplifying both sides of the AAT repeat is greater than 70%. we have obtained sequence information for 8 pairs of PCR primers and found the repeat sequence to amplify in all cases. Presently 1 of 6 is polymorphic as determined by survey of a panel of 10 unrelated female indi viduals. Examples of a highly polymorphic and a monomorphic STR are given in Fig. 1. POPULATION GENETIC FEATURES The establishment of reliable databases for STRs is operationally easier than for VNTR loci, since the method is PCR-based, alleles are distinguished by sequencing gel analysis, and the al lele number is generally smaller.

Eriksen Institute of forensic Genetics, University of Copenhagen frederik V's Vej 11, DK-2100 Copenhagen, Denmark INTRODUCTION Analysis for V-chromosome specific DNA sequences can be used to determine the sex of donors of biologica! samples containing nuclear DNA. In a case in which a child had died in a fire accident, and the abdomen was charred beyond recognition of the sex, we analysed DNA from an intact vertebra for the presence of the following V-specific sequences, using PCR: alpha satellite repeat (DVZ3), part of the testis determining gene (SRV), and part of the V-specific zinc-finger protein gene .

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Advances in Forensic Haemogenetics: 14th Congress of the International Society for Forensic Haemogenetics (Internationale Gesellschaft for forensische Hämogenetik e.V.), Mainz, September 18–21, 1991 by Prof. Dr. Christian Rittner, Dr. rer. nat. Peter M. Schneider (auth.), Prof. Dr. Christian Rittner, Dr. rer. nat. Peter M. Schneider (eds.)


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