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Since the rinsed vascular bed has to preserve all its characteristics several important physical and chemical parameters have to be considered in any vascular perfusion study. These are (i) perfusion pressure, (ii) flow rate, (iii) temperature, and (iv) chemical and physical composition of perfusates (ional composition, pH, osmolarity, viscosity). These parameters are, as indicated previously, combined by Poiseuille's law, whereby the vessel characteristics (nature, luminal diameter) are pregiven.
As in non-corroded cast specimens [61J specimens have to be fixed by immersion and processed as in tissue preparations for SEM inspection. Total maceration serves to remove all soft tissues, exposes the surface of the cast and enables a detailed examination. Parameters which influence maceration are: a) nature of the tissue to be macerated, b) chemical solutions used, c) duration of maceration, d) temperature of solutions, e) concentration of solutions used, f) frequency of renewing of solutions, and g) agitation of maceration solution.
In respect to the SEM inspection of vascular corrosion casts made from polymerizing resins (acrylates, epoxides) ultrahigh vacuum conditions may lead to outgasing and thus contaminate the microscope column. For further details on ion pumps we refer to  and product information sheets of companies manufacturing these pumps. 3. Focusing and Scanning System Condenser lenses. Most conventional scanning electron microscopes have two sets of condenser lenses. These lenses are electrostatic or electro magnetic lenses which further focus the electron beam according to their excitation.
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