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Extra resources for Current Protocols in Cytometry [All volumes]
Determination of linear fluorescence intensities from flow cytometric data accumulated with logarithmic amplifiers. Cytometry 3:251256. National Committee for Clinical Laboratory Standards (NCCLS). 1992. Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Peripheral Blood Lymphocytes. Tentative Guideline. H42-T. NCCLS, Villanova, Pennsylvania. A. R. 1995. Flow Cytometry for Clinical Laboratory Practice: Quality Assurance for Quantitative Immunophenotyping. Wiley-Liss, New York.
6 illustrate this approach. This method at least guarantees unambiguously that the instrument is able to resolve a certain low level of fluorescence. Making this approach quantitative requires not only attention to the relative numbers of unstained and dimly stained particles, but also some limits on the inherent particle fluorescence CV. By calibrating the unstained and dimly stained particles in MESF, one could have a method that would standardize and calibrate sensitivity. Spectral Overlap Compensation Measurement of fluorescence in a particular spectral range is not the same as measuring the fluorescence from a fluorochrome.
Y. Acad. Sci. 677:1-20. Darzynkiewicz, Z. 1993. The cell cycle: Application of flow cytometry in studies of cell reproduction. D. E. V. ) pp. 13-40. Williams and Wilkins, Baltimore. E. 1996. Quantitation and valence of an tibo dies bound to cells. ):125. G. 1990. Controls, standards, and histogram interpretation in DNA flow cytometry. Methods Cell Biol. 33:157-171. R. F. L. ) pp. 351-358. John Wiley & Sons, New York. G. and Nezel, M. 1978. Internal calibration to absolute values in flow-through particle size analysis.
Current Protocols in Cytometry [All volumes] by Wiley InterScience (Online service)